The Extraction of Fat from Liquid Milk Products

Introduction

Conventional methods
of fat extraction from liquid milk products are time and labor intensive, and require large amounts of hazardous solvents. Supercritical Fluid Extraction (SFE) using CO2 as a solvent is a solvent-free alternative
method for extraction and isolation of fat content from different milk-based nutrition products.

Equipment
 Applied Separations’ Spe-ed SFE Supercritical Extraction System

Materials
 Spe-ed™ Matrix (Catalog #7950)
 Ethanol (denatured)
 Carbon dioxide (instrument grade)

Method
Weigh 2g of sample into 5g of Spe-ed Matrix. Mix the milk sample and Spe-ed Matrix thoroughly and pour mixed sample into an
extraction vessel. Add 1mL of ethanol to the
extraction vessel. Place a preweighed collection vial onto the discharge tube and extract at specified conditions. Remove vial with fat extract and weigh.

Extraction Conditions
Sample: 2.0g
Pressure: 9000 psi
Temperature: 100ºC
CO2 Flow Rate: 3L/minute (gas)

Collection: preweighed vial
Extraction time: 25 min. dynamic

Results
Infant Formula Concentrate

Conclusions
Supercritical CO2 extractions of liquid milk products were accurate and precise when compared to the standard mojonnier extraction. Hazardous solvents were eliminated with significant savings in sample processing time.

References
1. AOAC Method 989.05
2. JAOAC 71, 898 (1988)